Number fluctuation spectroscopy of motile microorganisms

Number fluctuation spectroscopy of motile microorganisms.

A random-walk model of motility is used to predict the dynamics of fluctuations in the number of particles in a small observation volume. The results show that number fluctuations provide a measure of the mean swimming speed as well as the persistence length. Experimental light-scattering results are presented for three strains of Escherichia coli whose motion appears random-walk in nature. For...

The Influence of Sperm Morphology, Total Motile Sperm Count of Semen and the Number of Motile Sperm Inseminated in Sperm Samples on the Success of Intrauterine Insemination

Identification and characterization of pigmented photoreceptor-producing microorganisms using FTIR spectroscopy

The identification, differentiation and classification of microorganisms have been subjects of research for many years. Recently, Fourier transform infrared (FTIR) spectroscopy techniques have gained attention in the characterization and classification of microorganisms based on biochemical profiles and cell structure characteristics. In the present study, the characterization and differentiati...

Fluorescence fluctuation spectroscopy in subdiffraction focal volumes.

We establish fluorescence fluctuation spectroscopy (FFS) with nanoscale detection volumes generated by stimulated emission depletion. Our method applies fluorescence correlation spectroscopy and fluorescence intensity distribution analysis to extract molecular information about mobilities and fluorescence emission in solution. The combination of correlation analysis with that of photon intensit...

Cumulant analysis in fluorescence fluctuation spectroscopy.

A novel technique for the analysis of fluorescence fluctuation experiments is introduced. Fluorescence cumulant analysis (FCA) exploits the factorial cumulants of the photon counts and resolves heterogeneous samples based on differences in brightness. A simple analytical model connects the cumulants of the photon counts with the brightness epsilon and the number of molecules N in the optical ob...